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The effects of NaCl salinity (0 and 200 mM) and NH₄:NO₃ ratio (0:100, 25:75, 50:50 and 75:25) on the activities of antioxidant enzymes, viz. glutathione reductase (GR), superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and glutathione-S-transferase (GST), were investigated in leaves and roots of canola (Brassica napus L.). Salinity caused a reduction in plant biomass. The biomass production of plants at 75:25 NH₄:NO₃ was lower than that of plants at 0:100 NH₄:NO₃. The antioxidant enzymes exhibited higher activity in saline-treated plants. Changes in antioxidant enzyme activity caused by different NH₄:NO₃ ratios differed in leaves as well as in roots of canola. At 75:25 and 50:50 NH₄:NO₃ ratios plants showed higher CAT, GR and GST activity in leaves and roots, while POD and SOD activity was higher at 0:100 NH₄:NO₃.

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Effect of salinity and N sources on the activity of antioxidant enzymes in canola (Brassica napus L.)

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