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Author: João V.B. Souza ^1*, Érica S. Silva ², José R.P. Cavallazzi ³ and Agostinho da S. Sobrinho ⁴

Abstract

The aim of this work was to formulate a liquid medium with wheat bran for the production of Trametes versicolor laccase in an air-lift bioreactor. Uni- variated experiments were carried out in order to optimize the contents of CuSO₄ (0-2 mM), glucose (0-50 g/L), peptone (0-25 g/L) and fermentation parameters as pH (3-7), inoculum (40-360 mycelium plugs/L) and aeration (0-1 v/v/m). After the optimization an optimized experimentwas accomplished (pH 3, inoculum of 40 mycelium plugs/L, CuSO₄ concentration of 1.2 mM, 10 g/L of peptone and the aeration of 0.5 vvm) in an air- lift reactor. The highest activity (32,000 UI/L) was reached in the 10^th experimental day.

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Formulation of a liquid medium with wheat bran for the production of laccase by Trametes versicolor in an air-lift bioreactor

Abstract

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